OPTIMIZATION OF SOMATIC EMBRYOGENESIS CULTURE MEDIUM INDUCTION IN COTTON (Gossypium hirsutum L. CV. Y331B-R5) GROWTH IN CÔTE D’IVOIRE
Abstract
The development of an efficient protocol for somatic embryogenesis in cotton is an essential prerequisite for the adoption of genetic techniques transformation for varietal improvement. The present study aims to evaluate the responses to somatic embryogenesis of the Y331B-R5 cotton variety widely grown in Côte d'Ivoire. For this purpose, the hypocotyl of the vitroplants was used as an explant to initiate friable callus, which is used for the induction of somatic embryos. The effect of the medium compound on somatic embryogenesis was evaluated. For this purpose, four carbon sources (maltose; sucrose; fructose and glucose) at 30 g.L-1 were tested. Then, the effect of different concentrations (20; 30; 40 and 50 g.L-1) of the best carbon source was evaluated. Then, four amino acids (glutamine; casein hydrolysate; glycine and asparagine) were tested. Finally, phytohormones were added to induce embryos. The results showed that glucose at 30 g.L-1 induced the highest level of embryogenic cells (67.34 %). Amino acids tests showed that glutamine (0.5 mg.L-1) was more beneficial (47.31 %). Finally, hormones combination (0.1 mg L-1 2,4-D + 0.5 mg L-1 KIN) induced the highest rate (74.25 %). The MIE medium + 30 g.L-1 glucose + 0.1 mg L-1 2,4-D + 0.5 mg L-1 KIN was the most effective for the induction of somatic embryogenesis in Y331B-R5.
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References
Davidonis, G., H. & Hamilton, R., H. (1983). Plant regeneration from Callus tissue of Gossypium klotzchianum L. Plant Science Letter, 32, 89-93.
Declerck, V. & Korbon, S. S. (1986). Influence of growth regulators and carbon sources on induction growth and morphogenesis from leaf tissues of peach (Prunus persica L. Batsch). Journal of Horticumtural Sciences, 71(1), 49-50.
Finer, J. J. & McMullen, M. D. (1990). Transformation of cotton (Gossypium hirsutum L.) via particle bombardment. Plant Cell Report, 8, 586-589.
Finer, S. E. (1988). The Man on Horseback (2nd Edition Ed.). Boulder, Colorado: Westview Press.
Firoozabady, E., Heckert, M. & Gutterson, N. (2006). Transformation and regeneration of pineapple. Plant Cell, Tissue and Organe Culture, 4 1-16.
Gamborg, O., Miller, R. & Ojima, K., 1968. Nutriment requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50, 151-158.
Han, H. & Xi, T. (1989). Rapid propagation of lecttuce by embryos. Plant Physiology, 2, 17-20.
Haouala, F., farhat, N. & Chabchoub, L. (2010). Effets du type et de la position de l’explant sur l’induction de cals chez le gerbera (Gerbera jamesonii Bolus). Tropicultura, 28(1), 57-60.
ICTSD (Intertional Centre for Trade and Sustainable Development). 2010. Sustainable Development In International Intellectual Property Law – New Approaches From EU Economic Partnership Agreements. 45 p.
Komura, H., Chokyu, S., & Ikeda, Y. (1990). Micropropagation of Asparagus through somatic embryogenesis and plant regeneration from seedling. Bulletin. Hiroshima Prefect. Agricultural Experimental Station, 53, 43-50.
Koné, M. 2010. Développement de méthodes de régénération in vitro du voandzou [Vigna subterranea (l.) Verdc. (fabaceae)] et analyse de l’homogénéité variétale des plantules régénérées, Thèse de Doctorat, 214p.
Kouadio, J. Y., Mongomaké, K., Djè, Y., D’Almeida, M. A. & Zouzou, M. (2004). L’étiolement est un facteur d’induction de l’embryogenèse somatique au cours de la callogenèse chez deux variétés récalcitrantes de cotonnier (Gossypium hirsutum L.) cultivées en Côte d’Ivoire. Biotechnologie Agronomie Société et Environnement, 8(3), 155-162.
Kouadio, O. K. S. (2018). Effet de la composition du milieu de culture sur la régénération in vitro de l’ananas [Ananas comosus (L.) var. Cayenne lisse] par embryogenèse somatique indirecte et impact du stress salin sur les paramètres morphophysiologiques des vitroplants régénérés. Thèse de Doctorat, 206 p.
Kouadio, O. K. S., Yapo, E. S. S., Kouassi, K. M., Silue, O., Koffi, E. & Kouakou, T. H. (2017). Imporved callogenesis and somatic embryogenesis using amino acids plant growth regulators combination in pineapple [Ananas comosus (L.) merr. (Bromeliaceae)]. European Journal of Biotechnologie and Bioscience, 5(5), 06-16.
Kouakou, K. L. (2009). Optimisation de la production de plantules de deux espèces de rotin Laccosperma secundiflorum (P. Beauv) Kuntze et Eremospatha macrocarpa (G. Mann & Wendl.) H. Wendl commercialisées en Afrique tropicale. Thèse de Doctorat, 154 p.
Kouakou, T. H. (2003). Contribution à l’étude de l’embryogenèse somatique chez le cotonnier (Gossypium hirsutum L.) : évolution de quelques paramètres biochimiques au cours de la callogénèse et de cultures de suspensions cellulaires. Thèse de Doctorat, 144 p.
Kumar, M., singh, H., Shukla, A. K., Verma, P. C. & Singh, P. K. (2013). Induction and establishment of somatic embryogenesis in elite Indian cotton cultivar (Gossypium hirsutum L. cv Khandwa-2). Plant signaling & Behavior, 8(10), 26762-26767.
May, R. A., & Trigiano, R. N., (1991). Somatic embryogenesis and plant regeneration from leaves of Dendranthema grandifloro. Journal of American Society for Horticultural Science, 116 (2), 366-371.
Miranda, J. E., Rodrigues, S. M. M., De Almeida, R. P., Da Silva, C. A. D. & Togola, M. ( 2013). Reconnaissance de ravageurs et ennemies naturels pour les pays C-4. Embrapa Brasília, DF, Brésil, 25, 74 p
Murahige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco virus cultures. Physiology plant, 15, 473-497.
Najiba, B., Dou, E. & Abdelhadi, A. (2008). Effet du sucre sur l’embryogenèse somatique de l’olivier (Olea europaea L.) cv. «Picholine marocaine» Biotechnol. Agronomy and Society and Environ, 12(3), 245-250.
N'guessan, A. S. R., Ayolie, K., Yapo, S. E. S., Konan, Y. K. F., N’cho, A. L., N’goran, K. D., Kone, D., Kouadio, Y. J. & Kouakou, T. H. (2019). Induction of High-Frequency Callus with an Elite Cotton (Gossypium Hirsutum L.) from Côte d'Ivoire. Journal of Advances in Biotechnology, 8, 1104-1117.
Price, H. J. & Smith, R. H. (1979). Somatic embryogenesis in suspension cultures of gossypium klotzschianum Anderss. Planta, 145, 305-307.
Raju, C. S., Kathiravan, K., Aslam, A. & Shajahan, A. (2013). An efficient regeneration system via somatic embryogenesis in mango ginger (Curcuma amada Roxb.). Plant Cell, Tissue and Organ Culture, 112(3), 387-393.
Rangan, T. S., Zvala, T. & Ip, A. (1984). Somatic embryogenesis in gossypium klotzschianum L. in vitro, 20, 256 p.
Rathore, M. S., Paliwal, N., Anand, K. V. & Agarwal, P. K. (2015). Somatic embryogenesis and in vitro plantlet regeneration in Salicornia brachiata Roxb. Plant Cell, Tissue and Organ Culture, 120(1), 355-360.
Richter, G. (1993). Les glucides in Métabolisme des végétaux. Physiologie et brochure. Ed Presses Polytechniques et Universitaires. Romandes chap. IV, pp 189-220.
Robinson, P. J., Srivardhini, S. & Sasikumar, G. (2011). Somatic embryogenesis and plant regeneration from cotyledon tissue of Arachis hypogaea L. Research in Plant Biology, 1(3), 21-27.
Rout, G. R., Debata, B. K. & Das, P. (1991). Somatic embryongenesis in callus culture of Rosa hybryda L. cv. Landora. Plant Cell, Tissue and Organ Culture, 27(1), 65-69.
Sakhanokho, H. F., Zipf, A., Rajasekaran, K., Saha, S. & Sharma, G. C. (2001). Induction of highly embryogenic calli and plant regeneration in Upland (Gossypium hirsutum L.) and Pima (Gossypium barbadense L.) cotton. Crop Science, 41, 1235-1240.
Thiruwengadam, M., Mohamed, S., Yang, C. & Jayabalan, N. (2006). Development of an embryogenic suspension culture of bitter melon (Momordica charantia L). Scientia Horticulturae, 109, 123-129.
Trolinder, N. L. & Goodin, J. R. (1988). Somatic embryogenesis in cotton (Gossypium): Requirement for embryo development and plant regeneration. Plant Cell, Tissue Organe Culture, 12, 43-53.
Yapo, S. E .S. (2013). Propagation et régénération in vitro de l’ananas (ananas comosus var. comosus (l. merrill) coppens & leal) cultivé en côte d’ivoire et étude physicochimique des fruits issus des vitrocultures. Thèse de Doctorat, 151p.
Zouzou, M., Kouakou, T. H., Koné, M., Amani, N. G., & Kouadio, Y. J. (2008). Effect of genotype, explants, growth regulators and sugars on callus induction in cotton (Gossypium hirsutum L.). Australian Journal of Crop Science, 2(1), 1-9,
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