To evaluate antioxidant, cytotoxic, antibacterial activities of aerial parts ethanol extracts from Nepeta sibirica L. grown in Mongolia

  • Amarjargal Ayurzana Khovd state university
  • Bayarkhuu Batbayar Khovd state university, Mongolia
  • Irekhbayar Jambal National university Mongolia, Mongolia
  • Altantsetseg Shatar Institute of Chemistry and Chemical Technology, Mongolian Academy of Sciences
  • Oyuntsetseg Chultem Khovd state university, Mongolia
DOI: https://doi.org/10.53555/as.v6i10.3946
Keywords: Nepeta sibirica L.; essential oil; antimicrobial; antioxidant, and cytotoxic activities

Abstract

: Mongolia is rich in medicinal plants. In recent years, interest in plant-derived food additives has grown. Many Lamiaceae species have a characteristic scent or taste, which in many cases are the reason for their application in Mongolian traditional medicine. This study was aimed to evaluate antioxidant, cytotoxic activities of aerial parts ethanol extracts from Nepeta sibirica L. grown in Mongolia. The antioxidant and cytotoxic activities of the essential oil and ethanol crude extracts were determined by using DPPH and MTT assays. The ethanol extracts showed higher antioxidant activity than essential oil. The results clearly showed that the essential oil presented satisfactory cytotoxic activity against two human tumor cell lines HepG2 (human liver cancer cell line); AGS (human stomach cancer cell line). Our work revealed that the ethanol extracts and essential oil of Nepeta sibirica L. grown in Mongolia has potential as sources of new antioxidant, and cytotoxic compounds, respectively.

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References

1. H. Vandendool, P. D. Kratz. A generalization of the retention index system including linear temperature programmed gasliquid partition chromatography. J. Chromatogr. 1963, 11, 463-471.
2. D. Kalemba, A. Kunicka. Antibacterial and antifungal properties of essential oils. Curr. Med. Chem. 2003, 10, 813-829.
3. U. J. Salzer. The analysis of essential oils and extracts (oleoresins) from seasonings-a critical review. CRC Crit. Rev. Food Sci. Nutr. 1977, 9, 345-373.
4. A. Angioni, A. Barra, M. Arlorio, J. D. Coisson, M. T. Russo, F. F. Pirisi, M. Satta & P. Cabras. Chemical composition, plant genetic differences, and antifungal activity of the essential oil of Helichrysum italicumG. Don ssp. microphyllum (Willd) Nym. J. Agric Food Chem. 2003, 51, 1030-1034.
5. F. Senatore, N. A. Arnold & F. Piozzi. Chemical composition of the essential oil of Salvia multicaulis Vahl. var. simplicifolia Boiss. growing wild in Lebanon. J. Chromatogr. A. 2004, 1052, 237-240.
6. J. Sikkema, J. A. de Bont & B. Poolman. Mechanisms of membrane toxicity of hydrocarbons. Microbiol. Rev. 1995, 59, 201-222.
7. Daniela Rigano, Nelly Apostolides Arnold etc. Characterisation of the essential oil of Nepeta glomerata Montbret et Aucher ex Bentham from Lebanon and its biological activities. Natural Product Research. Vol. 25, No. 6, March 2011, 614–626.
8. V. I. Grubov. Key to the Vascular Plants of Mongolia. Nauka: Leningrad, Russia. 1982, 245-253.
9. S.Shatar, Sh. Altantsetseg. Essential oil composition of some plants cultivated in Mongolian climate. J. Essen. Oil Res. 2000, 12, 745-750.
10. S. Shatar. Моngolian fragrant herbs. Soyombo Press, UB, Mongolia. 1989, 14-16.
11. U. Ligaa, B. Davaasuren, N. Ninjil. Using the Mongolian medicinal herbs in eastern and western medicine. KSA Press, UB, Mongolia, 2005, 114.
12. R. P. Adams. Cedar Wood Oil - Analysis and properties. In: Modern Methods of Plant Analysis: Oils and Waxes. Springer-Verlag Berlin, Germany, 1991, 159-173.
13. Y. Massada. Analysis of Essential Oil by Gas Chromatography and Spectrometry. Wiley, New York, USA, 1976.
14. R. P. Adams. Identification of essential oils components by gas chromatography/mass spectroscopy. Carol Stream, Il, USA: Allured Publication Corporation, 2001.
15. W. Brand-Williams, M. E. Cuvelier, C. Berset. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 1995, 28, 25-30.
16. T. Mosman. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J.Immunol. Methods. 1983, 65, 55-63.
17. P. Molyneux. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 2004, 26, 211-219.
18. M. Martinez-Tome, A. Jimenez, S. Ruggieri, N. Frega, R. Strabbioli, & M. Murcia. Antioxidant properties of Mediterranean spices compared with common food additives. Journal of Food Protection, 2001, 64, 1412-1419.
19. Ts.Tsuruoka, S.Shatar, Sh.Altantsetseg, A.Amarjargal, The essential oil Mongolian Nepeta sibirica: a singlе component and its biological activities. Journal. Essential Oil. Res., 2012, Vol 24, No 6, .555-559.
20. European observatory on Health Systems and Policies. HiT profile in brief: Mongolia 2008. http://www.euro.who.int/__data/assets/pdf_file/0 004/98860/E90671sum.pdf?ua=1.
21. Lijana Dienaitėa , Milda Pukalskienėa , Ana A. Matias etc. Valorization of six Nepeta species by assessing the antioxidant potential, phytochemical composition and bioactivity of their extracts in cell cultures. Journal of Functional Foods (2018), https://doi.org/10.1016/j.jff.2018.04.004
22. Abbas Ali, Nurhayat Tabanca, Betul Demirci etc. Chemical Composition and Biological Activity of Essential Oils from Four Nepeta Species and Hybrids against Aedes aegypti (L.) (Diptera: Culicidae)., Rec. Nat. Prod. 10:2 (2016) 137-147.
23. Lijana Dienaitė, Milda Pukalskienė, Ana A.Matias, etc. Valorization of six Nepeta species by assessing the antioxidant potential, phytochemical composition and bioactivity of their extracts in cell cultures. Journal of Functional Foods (2018), https://doi.org/10.1016/j.jff.2018.04.004
Published
2020-11-03
How to Cite
Ayurzana, A., Batbayar, B., Jambal, I., Shatar, A., & Chultem, O. (2020). To evaluate antioxidant, cytotoxic, antibacterial activities of aerial parts ethanol extracts from Nepeta sibirica L. grown in Mongolia. IJRDO-Journal of Applied Science, 6(10), 121-131. https://doi.org/10.53555/as.v6i10.3946
Issue
Vol. 6 No. 10 (2020): IJRDO - Journal of Applied Science (ISSN: 2455-6653)
Section
Articles

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